🇵🇱 WOJEWÓDZKI INSPEKTORAT WETERYNARII W OPOLU PL

PCA AB 585 • Opole, Poland • 74 methods

Food & beveragesMicrobiologyEnvironment

WOJEWÓDZKI INSPEKTORAT WETERYNARII W OPOLU is a laboratory accredited by PCA under number AB 585 (Opole, Poland). Its accreditation scope covers 74 test methods in the following areas: Food & beverages, Microbiology, Environment. Test objects include: Feed, Raw meat of pigs and wild boars, Meat and meat products, Environmental samples from the food production and trade area E. Most frequently used techniques: Reference method, Gravimetric method, Culture method with biochemical and serological confirmation.

At a glance

  • Accreditation number: PCA AB 585
  • Methods in scope: 74
  • Types of test objects: 22
  • Techniques used: 30
  • City: Opole, Poland
Accreditation number
AB 585
Accreditation body
PCA
Address
ZAKŁAD HIGIENY WETERYNARYJNEJ, ul. Wrocławska 170, 45-836 Opole
City
Opole, Poland

What this laboratory tests (22)

Analytical techniques used (30)

Certificate information

Issue date
19.12.2025
Issue no.
25

Accreditation scope — test methods (74)

No. Test object Methodology / Parameters Technique Reference document
Test methods
1 Feed, Water Presence of rods of the genus, Salmonella Culture method with biochemical and serological confirmation PN-EN ISO 6579-1:2017-04 + A1:2020-09; White–Kauffmann–Le Minor scheme 2007
2 Feed Enterobacteriaceae count at 37°C Plate method (pour plate) PN-ISO 21528-2:2017-08
3 Feed Presence of animal protein DNA (ruminant / porcine / poultry) Real – time PCR method Commission Regulation (EU) No 51/2013 of 16.01.2013, Annex VI, cl. 2.2; | PB-01/Mp edition 1, issue of 15.10.2021
4 Meat and meat products Table salt content
Range (0,62 - 6,00) %
Potentiometric titration method PN-73/A-82112 | PN-73/A-82112/ Az1:2002
5 Meat and meat products Kjeldahl nitrogen content
Range (0,07 - 4,6) % nitrogen Protein content (calculated)
Titrimetric method PN-75/A-04018
6 Meat and meat products Nitrite content
Range (6 - 100) mg/kg Nitrate content Range (7 - 330) mg/kg
Spectrophotometric method PN-74/A-82114
7 Meat and meat products Total phosphorus content
Range (0,21 - 0,78) % P2O5 Polyphosphate content (calculated)
Gravimetric method PN-ISO 2294:1999
8 Meat and meat products Water content
Range (36,0 – 84,0) %
Gravimetric method PN-ISO 1442:2000
9 Meat and meat products Free fat content
Range (1,4 - 44,2) %
Gravimetric method PN-ISO 1444:2000
10 Feed Kjeldahl nitrogen content
Range (0,75 – 13,68) % nitrogen Crude protein content (calculated)
Titrimetric method Commission Implementing Regulation (EU) 2024/771 of 29 February 2024 Annex III, chapter C
11 Feed Crude fat content
Range (0,8 – 44,6) %
Gravimetric method Commission Implementing Regulation (EU) 2024/771 of 29 February 2024, Annex III, Chapter G
12 Feed Crude fibre content
Range (1,0 – 43,0) %
Gravimetric method Commission Implementing Regulation (EU) 2024/771 of 29 February 2024, Annex III, Chapter H
13 Feed Crude ash content
Range (0,19 – 87,70) %
Gravimetric method Commission Implementing Regulation (EU) 2024/771 of 29 February 2024 Annex III, chapter L
14 Feed Phosphorus content
Range (0,05 – 5,00) %
Spectrophotometric method Commission Implementing Regulation (EU) 2024/771 of 29 February 2024, Annex III, Chapter N
15 Feed Chloride content
Range (0,06 – 13,30) %
Potentiometric titration method Commission Implementing Regulation (EU) 2024/771 of 29 February 2024, Annex III, Chapter O
16 Feed Moisture content
Range (2,00 - 77,10) %
Gravimetric method Commission Implementing Regulation (EU) 2024/771 of 29 February 2024, Annex III, Chapter A
17 Feed Homogeneity (calculated) Instruction of the Chief Veterinary Officer No. GIWpr.0200.1.6.2020 of 12 March 2020
18 Feed Content of botanical impurities
Range (5 - 3500) mg/kg
Gravimetric method PB-12/CHPa edition 3 issue of 03.10.2019
19 Feed Presence of live pests Visual method PB-13/CHPa edition 3 issue of 03.10.2019
20 Feed Content of ash insoluble in hydrochloric acid
Range (0,04% - 7,36%)
Gravimetric method Commission Implementing Regulation (EU) 2024/771 of 29 February 2024, Annex III, Chapter M
21 Feed, agricultural products Fumonisin content Fumonisin B1,
Range (10-4000) μg/kg Fumonisin B2 Range (10-4000) μg/kg
High-performance liquid chromatography with fluorescence detection (HPLC-FLD) PB-15/CHPa edition 3 issue of 03.10.2019 developed on the basis of instruction PIW-PIB in Puławy
22 Feed, agricultural products Ochratoxin A content.
Range (0,9 - 150) μg/kg
High-performance liquid chromatography with fluorescence detection (HPLC – FLD) PB-03/CHPa edition 4 issue of 03.10.2019 developed on the basis of instruction PIW-PIB in Puławy
23 Feed, agricultural products Deoxynivalenol content
Range (25 - 5000) μg/kg
High-performance liquid chromatography with diode array detection (HPLC-DAD) PB-07/CHPa edition 4 issue of 03.10.2019 developed on the basis of instruction PIW-PIB in Puławy
24 Feed, agricultural products Zearalenone content
Range (8 - 3000) μg/kg
High-performance liquid chromatography with fluorescence detection (HPLC – FLD) PB-08/CHPa edition 4 issue of 03.10.2019 developed on the basis of instruction PIW-PIB in Puławy
25 Feed, agricultural products Metal content
calcium (0,24 – 455) g/kg copper (3,0 -18000) mg/kg zinc (14 – 62171) mg/kg sodium (0,076 – 102) g/kg potassium (0,05 – 49) g/kg iron (4,0 – 72000) mg/kg manganese (2,6 – 50000) mg/kg magnesium (0,01-74) g/kg
Flame atomic absorption spectrometry (FAAS) PN-EN ISO 6869:2002
26 Feed Starch content
Range (1,30 – 93,70 )%
Polarimetric method Commission Implementing Regulation (EU) 2024/771 of 29 February 2024, Annex III, Chapter K
27 Feed Sugar content
Range (1,00 – 59,00) % Sucrose content (calculated)
Titrimetric method (Luff –Schoorl) Commission Implementing Regulation (EU) 2024/771 of 29 February 2024 Annex III, chapter I
28 Poultry feed Estimation of energy value (calculated) Commission Implementing Regulation (EU) 2024/771 of 29 February 2024 Annex VII
29 Feed Content of packaging residues
Range (0,05 – 1,00)%
Gravimetric method PB-17/CHPa edition 2, issue of 03.10.2019, developed on the basis of the KLP Instruction in Lublin
30 Feed Physical impurity content
Range (0,002 – 1,000)%
Gravimetric method PB-17/CHPa edition 2, issue of 03.10.2019, developed on the basis of the KLP Instruction in Lublin
31 Feed E Presence of processed animal protein Microscopic method Legal regulations
32 Raw milk Somatic cell count Microscopic method PN-EN ISO 13366-1:2009 +AC:2009+Ap1:2009
33 Food E Microorganism count Plate method (pour plate) Standards
34 Food E Presence and identification of microorganisms Culture method with biochemical and serological confirmation Standards
35 Food E Presence of microorganisms Culture method with biochemical confirmation Standards
36 Food E Microorganism count Plate method (spread plate) Standards
37 Environmental samples from the food production and trade area E Microorganism count Plate method (pour plate) Standards
38 Environmental samples from the food production and trade area E Presence and identification of microorganisms Culture method with biochemical and serological confirmation Standards
39 Environmental samples from the food production and trade area E Presence of microorganisms Culture method with biochemical confirmation Standards
40 Environmental samples from the food production and trade area E Microorganism count Plate method (spread plate) Standards
41 Environmental samples from the food production and trade area E Presence of microorganisms Culture method with biochemical and microscopic confirmation Standards
42 Samples taken at the primary production stage, including biological material: surface swabs, disinfection efficacy swabs, boot swabs, liners from transport containers, dust, faeces, chicks, internal organs of animals, chick down, meconium, dead-in-shell embryos, hatching eggs Presence and identification of rods, Salmonella spp. Culture method with biochemical and serological confirmation PN-EN ISO 6579-1:2017-04 + A1:2020-09 ISO/TR 6579-3:2014
43 Brain tissue Presence of lyssavirus antigen Direct immunofluorescence method (IF) Instruction of the Chief Veterinary Officer No. GIWpr-02010-3/2018 of 7 February 2018
44 Bone sections Presence of tetracycline marker (TC) Microscopic method Instruction of the Chief Veterinary Officer No. GIWpr-02010-36/2016 of 12 December 2016
45 Bees, hive debris, brood, honey, food stores, wax Presence of Paenibacillus larvae Culture method with biochemical and microscopic confirmation Instruction of the Chief Veterinary Officer No. GIWpr 02010-23/2016 of 16 August 2016
46 Brain tissue Presence of lyssavirus Lyssavirus isolation in mouse neuroblastoma cell culture method (RTCIT) Instruction of the Chief Veterinary Officer No. GIWpr-02010-38/2016 of 12 December 2016
47 Blood serum, body cavity fluid Presence of antibodies against rabies virus Enzyme immunoassay method (ELISA) Instruction of the Chief Veterinary Officer No. GIWpr-02010-35/2016 of 12 December 2016 / test manufacturer's instructions
48 Bees, brood, hive debris Presence of Varroa destructor mites Microscopic method Instruction of the Chief Veterinary Officer No. GIWpr 02010-22/2016 of 16 August 2016
49 Biological material of animal origin: brain tissue, internal organs, swabs from the reproductive tract Presence and identification of Listeria spp. Culture method with biochemical confirmation Instruction of the Chief Veterinary Officer No. GIWpr-02010-7/2018 of 31 August 2018
50 Bee brood Presence of Melissococcus plutonius Culture method with microscopic confirmation Instruction of the Chief Veterinary Officer No. GIWpr-02010-24/2016 of 16 August 2016
51 Blood serum of cattle and small ruminants, pigs Presence of antibodies against Brucella spp. Acidified plate agglutination test (OKAP) Instruction of the Chief Veterinary Officer No. 27/2003 of 25 June 2003, ref. GIW z VII.420/lab – 4/2003
52 Blood serum of cattle and pigs Presence of antibodies against Brucella spp. Tube agglutination test (OA) Instruction of the Chief Veterinary Officer No. 26/2003 of 25 June 2003, No. GIW z VII.420/lab – 3/2003
53 Blood serum of cattle and pigs Presence of antibodies against Brucella spp. Complement fixation test method (OWD) Instruction of the Chief Veterinary Officer No. BP.0200.1.3.2022 of 30 August 2022
54 Animal blood serum E Presence of antibodies ELISA method Instructions of the Chief Veterinary Officer test procedures developed on the basis of the test manufacturer's instructions
55 Feed, Food Radionuclide activity concentration: 137Cs
Range (0,20-4806,01) Bq/kg
Gamma-ray spectrometry method PN-EN ISO 20042:2022-01
56 Feed, Food Radionuclide activity concentration: 134Cs
Range (0,20 – 406,3) Bq/kg
Gamma-ray spectrometry method PN-EN ISO 20042:2022-01
57 Feed E Genetic modification DNA content Real-time PCR method Standards, standardised documents ◄
58 Feed E Presence of DNA of genetic modification PCR / real-time PCR method Standards, standardised documents ◄
59 Raw meat of pigs and wild boars Presence of trichinae (Trichinella) by the magnetic stirring method of digestion of a pooled sample. Reference method PN-EN ISO 18743:2015-11+A1:2024-02 | Instruction of the Chief Veterinary Officer No. BP.0200.1.8.2024 of 8 May 2024
60 Raw meat of pigs and wild boars Presence of trichinae (Trichinella) by the magnetic stirring method of digestion of a pooled sample. Reference method PN-EN ISO 18743:2015-11+A1:2024-02 | Instruction of the Chief Veterinary Officer No. BP.0200.1.8.2024 of 8 May 2024
61 Raw meat of pigs and wild boars Presence of trichinae (Trichinella) by the magnetic stirring method of digestion of a pooled sample. Reference method PN-EN ISO 18743:2015-11+A1:2024-02 | Instruction of the Chief Veterinary Officer No. BP.0200.1.8.2024 of 8 May 2024
62 Raw meat of pigs and wild boars Presence of trichinae (Trichinella) by the magnetic stirring method of digestion of a pooled sample. Reference method PN-EN ISO 18743:2015-11+A1:2024-02 | Instruction of the Chief Veterinary Officer No. BP.0200.1.8.2024 of 8 May 2024
63 Raw meat of pigs and wild boars Presence of trichinae (Trichinella) by the magnetic stirring method of digestion of a pooled sample. Reference method PN-EN ISO 18743:2015-11+A1:2024-02 | Instruction of the Chief Veterinary Officer No. BP.0200.1.8.2024 of 8 May 2024
64 Raw meat of pigs and wild boars Presence of trichinae (Trichinella) by the magnetic stirring method of digestion of a pooled sample. Reference method PN-EN ISO 18743:2015-11+A1:2024-02 | Instruction of the Chief Veterinary Officer No. BP.0200.1.8.2024 of 8 May 2024
65 Raw meat of pigs and wild boars Presence of trichinae (Trichinella) by the magnetic stirring method of digestion of a pooled sample. Reference method PN-EN ISO 18743:2015-11+A1:2024-02 | Instruction of the Chief Veterinary Officer No. BP.0200.1.8.2024 of 8 May 2024
66 Raw meat of pigs and wild boars Presence of trichinae (Trichinella) by the magnetic stirring method of digestion of a pooled sample. Reference method PN-EN ISO 18743:2015-11+A1:2024-02 | Instruction of the Chief Veterinary Officer No. BP.0200.1.8.2024 of 8 May 2024
67 Raw meat of pigs and wild boars Presence of trichinae (Trichinella) by the magnetic stirring method of digestion of a pooled sample. Reference method PN-EN ISO 18743:2015-11+A1:2024-02 | Instruction of the Chief Veterinary Officer No. BP.0200.1.8.2024 of 8 May 2024
68 Raw meat of pigs and wild boars Presence of trichinae (Trichinella) by the magnetic stirring method of digestion of a pooled sample. Reference method PN-EN ISO 18743:2015-11+A1:2024-02 | Instruction of the Chief Veterinary Officer No. BP.0200.1.8.2024 of 8 May 2024
69 Raw meat of pigs and wild boars Presence of trichinae (Trichinella) by the magnetic stirring method of digestion of a pooled sample. Reference method PN-EN ISO 18743:2015-11+A1:2024-02 | Instruction of the Chief Veterinary Officer No. BP.0200.1.8.2024 of 8 May 2024
70 Raw meat of pigs and wild boars Presence of trichinae (Trichinella) by the magnetic stirring method of digestion of a pooled sample. Reference method PN-EN ISO 18743:2015-11+A1:2024-02 | Instruction of the Chief Veterinary Officer No. BP.0200.1.8.2024 of 8 May 2024
71 Raw meat of pigs and wild boars Presence of trichinae (Trichinella) by the magnetic stirring method of digestion of a pooled sample. Reference method PN-EN ISO 18743:2015-11+A1:2024-02 | Instruction of the Chief Veterinary Officer No. BP.0200.1.8.2024 of 8 May 2024
72 Raw meat of pigs and wild boars Presence of trichinae (Trichinella) by the magnetic stirring method of digestion of a pooled sample. Reference method PN-EN ISO 18743:2015-11+A1:2024-02 | Instruction of the Chief Veterinary Officer No. BP.0200.1.8.2024 of 8 May 2024
73 Raw meat of pigs and wild boars Presence of trichinae (Trichinella) by the magnetic stirring method of digestion of a pooled sample. Reference method PN-EN ISO 18743:2015-11+A1:2024-02 | Instruction of the Chief Veterinary Officer No. BP.0200.1.8.2024 of 8 May 2024
74 Raw meat of pigs and wild boars Presence of trichinae (Trichinella) by the magnetic stirring method of digestion of a pooled sample. Reference method PN-EN ISO 18743:2015-11+A1:2024-02 | Instruction of the Chief Veterinary Officer No. BP.0200.1.8.2024 of 8 May 2024

Reference standards (51)

Instruction of the Chief Veterinary Officer No. 26/2003 of 25 June 2003, No. GIW z VII.420/lab – 3/2003
Instruction of the Chief Veterinary Officer No. 27/2003 of 25 June 2003, ref. GIW z VII.420/lab – 4/2003
Instruction of the Chief Veterinary Officer No. BP.0200.1.3.2022 of 30 August 2022
Instruction of the Chief Veterinary Officer No. GIWpr-02010-3/2018 of 7 February 2018
Instruction of the Chief Veterinary Officer No. GIWpr-02010-35/2016 of 12 December 2016 / test manufacturer's instructions
Instruction of the Chief Veterinary Officer No. GIWpr-02010-36/2016 of 12 December 2016
Instruction of the Chief Veterinary Officer No. GIWpr-02010-38/2016 of 12 December 2016
Instruction of the Chief Veterinary Officer No. GIWpr.0200.1.6.2020 of 12 March 2020
Instruction of the Chief Veterinary Officer No. BP.0200.1.8.2024 of 8 May 2024
Instruction of the Chief Veterinary Officer No. GIWpr 02010-22/2016 of 16 August 2016
Instruction of the Chief Veterinary Officer No. GIWpr 02010-23/2016 of 16 August 2016
Instruction of the Chief Veterinary Officer No. GIWpr-02010-24/2016 of 16 August 2016
Instruction of the Chief Veterinary Officer No. GIWpr-02010-7/2018 of 31 August 2018
Instructions of the Chief Veterinary Officer test procedures developed on the basis of the test manufacturer's instructions
Standards
Standards, standardised documents ◄
PB-01/Mp edition 1, issue of 15.10.2021
PB-03/CHPa edition 4 issue of 03.10.2019 developed on the basis of instruction PIW-PIB in Puławy
PB-07/CHPa edition 4 issue of 03.10.2019 developed on the basis of instruction PIW-PIB in Puławy
PB-08/CHPa edition 4 issue of 03.10.2019 developed on the basis of instruction PIW-PIB in Puławy
PB-12/CHPa edition 3 issue of 03.10.2019
PB-13/CHPa edition 3 issue of 03.10.2019
PB-15/CHPa edition 3 issue of 03.10.2019 developed on the basis of instruction PIW-PIB in Puławy
PB-17/CHPa edition 2, issue of 03.10.2019, developed on the basis of the KLP Instruction in Lublin
PN-73/A-82112
PN-73/A-82112/ Az1:2002
PN-74/A-82114
PN-75/A-04018
PN-EN ISO 13366-1:2009 +AC:2009+Ap1:2009
PN-EN ISO 18743:2015-11+A1:2024-02
PN-EN ISO 20042:2022-01
PN-EN ISO 6579-1:2017-04 + A1:2020-09 ISO/TR 6579-3:2014
PN-EN ISO 6579-1:2017-04 + A1:2020-09; White–Kauffmann–Le Minor scheme 2007
PN-EN ISO 6869:2002
PN-ISO 1442:2000
PN-ISO 1444:2000
PN-ISO 21528-2:2017-08
PN-ISO 2294:1999
Legal regulations
Commission Regulation (EU) No 51/2013 of 16.01.2013, Annex VI, cl. 2.2;
Commission Implementing Regulation (EU) 2024/771 of 29 February 2024, Annex III, Chapter A
Commission Implementing Regulation (EU) 2024/771 of 29 February 2024 Annex III, chapter C
Commission Implementing Regulation (EU) 2024/771 of 29 February 2024, Annex III, Chapter G
Commission Implementing Regulation (EU) 2024/771 of 29 February 2024, Annex III, Chapter H
Commission Implementing Regulation (EU) 2024/771 of 29 February 2024 Annex III, chapter I
Commission Implementing Regulation (EU) 2024/771 of 29 February 2024, Annex III, Chapter K
Commission Implementing Regulation (EU) 2024/771 of 29 February 2024 Annex III, chapter L
Commission Implementing Regulation (EU) 2024/771 of 29 February 2024, Annex III, Chapter M
Commission Implementing Regulation (EU) 2024/771 of 29 February 2024, Annex III, Chapter N
Commission Implementing Regulation (EU) 2024/771 of 29 February 2024, Annex III, Chapter O
Commission Implementing Regulation (EU) 2024/771 of 29 February 2024 Annex VII

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